Reverse phase High Pressure Liquid Chromatography for measuring hydrolysis of ester Essay Example for Free

Reverse phase High Pressure Liquid Chromatography for measuring hydrolysis of ester Essay Reverse-Phase High performance chromatography consists of any method of chromatography that utilizes a non-polar phase. In the early part of 1970s, non-modofied alumina or silica which has a greater attraction for polar elements and hydrophilic surface chemistry was used for majority of the liquid chromatography. Because of this, this method was said to be normal†. The elution order was however reversed with the introduction of a covalently bonded alkyl chain which reinforces the surface. In this method, the first to be eluted are the polar compounds while there is retention of the non-polar compounds hence the procedure is termed reversed phase Method validation It’s important to validate all various HPLC methods. Evaluation of suitability may not be necessary when methods from pharmacopoeia’s are utilizes given that the analyses are carried out with respect to the technique’s intended use. However, in situation where drug composition is being modified, a reevaluation of suitability of the HPLC method to its new intended use is necessary (Nagels etal, 2002). The variables tested in the validation process as stated by FDA, ICH and USP as well as other health bodies include: Accuracy, linearity, limit of quantitation, limit of detection specificity relativity, reproducibility precision which includes repeatability, intermediate precision as well as ruggedness/reproducibility (Amersham, 1999). Role of rp-HPLC method for the measuring hydrolysis of ester Reverse phase high pressure liquid chromatography can be used in separation quantitation of free fatty acids as well as its methyl esters obtained from tissue phosphopids of mineral. In this method, mixtures of water acetonitrile are utilized for elution of esters. For free acids to separate, aqueous phosphoric acid substitute for water. Quantification detection of unsaturated compounds are done by absorption at 192nm . After methyl esters have been prefractionated based on its unsaturation by argentation TLC, there is total rapid separation by elution with CH3CN. Reverse phase HPLC can both be applied as preparative analytic method as well as in biochemical separation and purification. Precision In rp-HPLC, precision is assessed by injection of several standards followed by measurement of the variability of the results. Three subcategories are obtained from the measured standard deviation . To assess repeatability, the operator executes the analysis in a laboratory over a short period of time. Then, the relative standard deviation is calculated from 5 or 6 determinates of two dissimilar matrices at 1 or 2 different concentrations. The ICH describes intermediate precision as long term variability this is estimated by comparison of several results of a single laboratory over several weeks. Variations of results gotten by different instruments, different batches of column and from operators with materials from dissimilar supplies are reflected in intermediate precision (Amersham, 1999) It is important to validate intermediate precision in rp-HPLC so that verification of same results being produced by same method in same laboratory following the phase of development could be possible. Reproducibility otherwise called ruggedness is simply inter-laboratory precision . It’s aim is to ensure that different laboratories will have same results using the method. Variables that affect the reproducibility of rp-HPLC include the varying operator experience, varying ages of equipment, differential supplies of columns, variation in humidity room temperature, different characteristics of equipment such as the delay capacity of HPLC system (Lebendiker, 2006). Accuracy is the degree in which test outcomes are proximate to the true value. This is obtained from outcome of quantitative assessment of a known sample. Whatever is measured is then compared to the known amount. Selectivity specificity These are usually interchanged . Selectivity of analytical method like rp-HPLC refers to its capability despite the presence of interference, to still be able to measure accurately (Guzzetta, 2010). Such interference includes enantiomers, degradation products and excipients. When selectivity of a method is verified, it becomes â€Å"Stability Indicating Method†. Limit of detection This refers to the least amount or concentration of analyte in a chromatographic sample that may not be quantifiable but is detectable. In rp-HPLC, the limit of detection is that amount which after injected gives a maximum height of 2 to 3 times as raised as the reference noise level. Limit of quantitation refers to the least amount injected that results in precise measurement. In rp-HPLC, maximum heights of about ten to twenty times greater than reference noise are usually required at precision of less than 10-15% RSD between intervals of results. Linearity With injection of three to five series of four or more standards with spanning concentration of 80-120% expected range, the linearity of a rp-HPLC analytical method can be determined (Amersham, 1999). Whether directly or by calculation, it is expected that the response should be in proportion to the analytes’ concentration. The intercept of linear regression formula applied to these results should not substantially differ from zero. An intercept of significant non-zero should be followed by validation that there is no impact on method accuracy (Alveldano etal, 2010). Principle This operates of the separation of substances based on hydrophobic binding between the stationary/immobile hydrophobic ligand (called the stationary phase) and the solute in the mobile phase (Amersham, 1999). The binding interaction is presumed to be due to favorable entropy effect. The original mobile phase conditions in rp-HPLC are aqueous implying a highly organized water structure around stationary ligand solute molecule . The available area exposed to solvent becomes diminished with binding of solute to the stationary hydrophobic ligand. With this, there is decrease in organization of water structure increase system entropy (Amersham, 1999). In summary, rp-HPLC is based on â€Å"adsorption of hydrophobic molecules onto a hydrophobic solid support in a polar mobile phase†. Reverse phase HPLC has also been utilized in most analytical methods.

The Philosophy of Teaching Essay -- Education Teachers School Essays

My Philosophy of Teaching Philisophical teaching stratigies include the following disciplines, essentialism, existentialism, and many others. Of these stratigies the philisophicla approaches each include a distinct direction in the style and type of learning taking place. Lessons should include these philisophical teaching stratigies within there designed properties. Although all of the stratigies may be able to incorporate all learning disciplines and learning styles a lesson does not need to contain the entire array of philisophical stratigies. Informitive lessons may take place without all stratigies invovled. William Bagley's essentialsim is one of the philisophical styles which I find to be a large protion of the melting pot which I agree to. The essentialsim style is a basic philisophcial idea pertaining largely to math, science, history, and literature. This style of basic fundamentals can create a strong backbone of learning and knowledge. The essentialsim philosophy not only provides the student with a background in many disciplines and views, it also offers the student more. The student also gains by essentialism due to its influence of character. This building and concentration of character allow students not only to gain knowledge , but respect and confidense. Although this style is not my soul reliance. Essentialsim also focus's on the teacher more heavily. The idea follows the style of a larger group learning enviornment. This mass style learning allows for less individual attentiona nd intsruction. Not all students learn in mass groups and not all students require personal attention, thus the blend off other disciplined styles must be required. Essen... ... usefullness which it provides students. The curriculum is advantageous to the students as well as society, becuase it allows them to learn from previous mistakes and achievments and install those into their lives. My future educational goals after graduation are somewhat unorthadox in style compared to that of the average post graduate.Many graduates seek to continue their higher education at an institution where a masters or phd can be earned. I plan to recieve my post graduate studies at an institution of much lower levels. The curriculum I plan on teaching will provide a prfound and invaluable post graduate studies program for me. The actions of daily teaching coupled with the problem solving situations of daily school activities will engrain in me an understanding and furthured education which no higher educational institution can provide.

Mental Illness

The social construction of mental illness Key Words * Career: The gradual change in people as a response to a label e. g. mental patient. * Learned Helplessness: learning how to be dependent. * Life-course model: suggests that the accumulation of social events experienced over a whole lifetime, not just individual important events, influence people and their mental state. * Presenting culture: a term used by Goffman to refer to how people like to portray themselves to others. * Schizophrenia: a form of mental illness where people are unable to distinguish their own feelings and perceptions from reality. Self-Fulfilling prophecy: predictions about the behaviour of social groups that come true as a result of positive or negative labelling. * Social Capital: refers to a network of social contacts. * Social constructionism: the approach which suggests that mental illness exists because people believe that it does. * Social realism: a sociological approach which suggests that mental illne ss does really exist. Summary Mental illness is the less fortunate twin to physical illness. The NHS is not funding enough support for mental health patients and the attention paid to it is minimal.Mental health is a major problem in society with one in seven people claiming to have had mental health problems at some point in their lives. Social Trends 2007 (Self and Zealey 2007) said that about one in six British people aged 16 to 74 reported experiencing a neurotic disorder in the seven days before a national survey on mental health. When looking at which group is most likely to suffer from high rates of mental illness, the poorest and most excluded are majorly overrepresented. Defining mental illness Social Realism: A general term used to describe the approaches of sociologists who accept that there are distinctive sets of abnormal behaviour that cause distress to individuals and those around them. * Pilgrim and Rogers (1999) accept that, at different times and in different cultu res, there are variations in what is considered as mental illness. * Although mental illness may have different names and sometimes not be recognized, it does actually exist as a real condition. * Similar to the bio-medical approach which believes that symptoms can be scientifically diagnosed and categorized.They see treatment as allopathic (cure orientated through the use of drugs, shock treatment and surgery etc. * They recommend that sufferers be isolated from wider society. Social constructionism: * Have been very influential in sociological approaches to mental illness and start from the argument that what is considered as normal varies over time and from society to society. * Greater extremes of behaviour have been seen as normal in some societies and symptoms of madness in others. Labelling perspective: Labelling theory examines how the labelling of mental illness occurs in the first place and what effects it has on those who are labelled. * Thomas Szasz (1973) argues that th e label ‘mental illness’ is simply a convenient way to deal with behaviour that people find disruptive. He is particularly critical of psychiatrists for diagnosing children with ADHD and calling it a disease. He says that giving a child a drug for a mythical disease is a form of physical child abuse because the child has no say in the matter. Labelling theory therefore rests firmly upon a social constructionist definition of mental illness. The effects of labelling * Scheff (1966) said that whether someone becomes labelled or not is determined by the benefits those others might gain by labelling the person ‘mentally ill’. So, those who become a nuisance are far more likely to become diagnosed as mentally ill as someone who causes no problems. * Once labelled, there are a number of negative consequences for the person because it is then assumed that all their behaviour is evidence of their mental state. Erving Goffman (1961) followed the careers of people wh o were genuinely defined as being mentally ill. He suggested that once in an institution people are stripped of their presenting culture. Criticisms of the labelling perspective * Gove (1982) suggests that the vast majority of people who receive treatment for mental illness actually have serious problems before they are treated so the argument that the labels cause the problems is wrong. It may explain the responses of others to the mentally ill, but cannot explain the causes of the illness.Foucault’s perspective on mental illness: * He explains the growth in the concept of mental illness by placing it in the context if the changing ways of thinking and acting which developed in the early 18th century. * During the enlightenment more traditional ways of thinking were gradually replaced by more rational and disciplined ways of thinking. He argues that as rationality developed into the normal way of thinking, irrationality became to be perceived as deviant. * Having mad people in asylums isolated mad people away from the majority of the population.They symbolized the fact that madness or irrationality was marked out as behaviour that is no longer acceptable. Structuralist perspectives on mental illness: * Virde (1977) explained the fact that some ethnic minorities are more likely to develop mental health problems by arguing that the sorts of pressures and stresses that can cause people to develop mental illness are more often experienced by people in an ethnic minority. * Nazroo is critical of this approach. He points out that people of the Bangladeshi origin who are victims of racism have lower levels of mental illness than the general population.He concludes that mental illness cannot just be caused by racism and deprivation. * Brown et al (1995) explained that women are more likely to lead stressful lives because they have the dial burden and triple shift. * Labelling theorists (Chelser 1972), say that women are more likely to be seen as mentally ill b ecause the defining of illness is mainly done by males. * Link and Phelan (1995) reviewed all the evidence and concluded that research pointed out a relationship between low levels of deprivation and mental illness.Social capital (Putnam 2000) argued that people who have extensive and strong levels of social networks are more likely to be ‘happier’ than those who don’t. Check Your Understanding: 1) The two sociological approaches to explaining mental illness are social realist and social constructionist. 2) Social realist bases itself on the idea that there are distinctive sets of abnormal behaviour that should be treated; they are very similar to the bio-medical approach.Social constructionist believes that definitions of mental illness can vary over time and in different cultures. 3) Labelling helps us understand that some people may live up to the label they have been given and can strip mental health patients of any self-dignity as they have no say in what ha ppens to them as they have been given the label of someone who is mad. 4) A structural explanation is closely tied to the social realist definition of mental illness; they accept the reality of mental illness and set out to discover what social factors help cause them. ) Busfield says that it is true that some groups are more likely to find their behaviour defined as mental illness, compared to the behaviour of other groups however al of those groups experience higher levels of stress so mental illness would be higher. 6) They are more likely to show cultural characterises that are not seen as normal in a wider society. 7) They use the idea that women have more responsibilities than men and that the defining of mental illness tends to be dominated by male health professionals. Mental Illness The social construction of mental illness Key Words * Career: The gradual change in people as a response to a label e. g. mental patient. * Learned Helplessness: learning how to be dependent. * Life-course model: suggests that the accumulation of social events experienced over a whole lifetime, not just individual important events, influence people and their mental state. * Presenting culture: a term used by Goffman to refer to how people like to portray themselves to others. * Schizophrenia: a form of mental illness where people are unable to distinguish their own feelings and perceptions from reality. Self-Fulfilling prophecy: predictions about the behaviour of social groups that come true as a result of positive or negative labelling. * Social Capital: refers to a network of social contacts. * Social constructionism: the approach which suggests that mental illness exists because people believe that it does. * Social realism: a sociological approach which suggests that mental illne ss does really exist. Summary Mental illness is the less fortunate twin to physical illness. The NHS is not funding enough support for mental health patients and the attention paid to it is minimal.Mental health is a major problem in society with one in seven people claiming to have had mental health problems at some point in their lives. Social Trends 2007 (Self and Zealey 2007) said that about one in six British people aged 16 to 74 reported experiencing a neurotic disorder in the seven days before a national survey on mental health. When looking at which group is most likely to suffer from high rates of mental illness, the poorest and most excluded are majorly overrepresented. Defining mental illness Social Realism: A general term used to describe the approaches of sociologists who accept that there are distinctive sets of abnormal behaviour that cause distress to individuals and those around them. * Pilgrim and Rogers (1999) accept that, at different times and in different cultu res, there are variations in what is considered as mental illness. * Although mental illness may have different names and sometimes not be recognized, it does actually exist as a real condition. * Similar to the bio-medical approach which believes that symptoms can be scientifically diagnosed and categorized.They see treatment as allopathic (cure orientated through the use of drugs, shock treatment and surgery etc. * They recommend that sufferers be isolated from wider society. Social constructionism: * Have been very influential in sociological approaches to mental illness and start from the argument that what is considered as normal varies over time and from society to society. * Greater extremes of behaviour have been seen as normal in some societies and symptoms of madness in others. Labelling perspective: Labelling theory examines how the labelling of mental illness occurs in the first place and what effects it has on those who are labelled. * Thomas Szasz (1973) argues that th e label ‘mental illness’ is simply a convenient way to deal with behaviour that people find disruptive. He is particularly critical of psychiatrists for diagnosing children with ADHD and calling it a disease. He says that giving a child a drug for a mythical disease is a form of physical child abuse because the child has no say in the matter. Labelling theory therefore rests firmly upon a social constructionist definition of mental illness. The effects of labelling * Scheff (1966) said that whether someone becomes labelled or not is determined by the benefits those others might gain by labelling the person ‘mentally ill’. So, those who become a nuisance are far more likely to become diagnosed as mentally ill as someone who causes no problems. * Once labelled, there are a number of negative consequences for the person because it is then assumed that all their behaviour is evidence of their mental state. Erving Goffman (1961) followed the careers of people wh o were genuinely defined as being mentally ill. He suggested that once in an institution people are stripped of their presenting culture. Criticisms of the labelling perspective * Gove (1982) suggests that the vast majority of people who receive treatment for mental illness actually have serious problems before they are treated so the argument that the labels cause the problems is wrong. It may explain the responses of others to the mentally ill, but cannot explain the causes of the illness.Foucault’s perspective on mental illness: * He explains the growth in the concept of mental illness by placing it in the context if the changing ways of thinking and acting which developed in the early 18th century. * During the enlightenment more traditional ways of thinking were gradually replaced by more rational and disciplined ways of thinking. He argues that as rationality developed into the normal way of thinking, irrationality became to be perceived as deviant. * Having mad people in asylums isolated mad people away from the majority of the population.They symbolized the fact that madness or irrationality was marked out as behaviour that is no longer acceptable. Structuralist perspectives on mental illness: * Virde (1977) explained the fact that some ethnic minorities are more likely to develop mental health problems by arguing that the sorts of pressures and stresses that can cause people to develop mental illness are more often experienced by people in an ethnic minority. * Nazroo is critical of this approach. He points out that people of the Bangladeshi origin who are victims of racism have lower levels of mental illness than the general population.He concludes that mental illness cannot just be caused by racism and deprivation. * Brown et al (1995) explained that women are more likely to lead stressful lives because they have the dial burden and triple shift. * Labelling theorists (Chelser 1972), say that women are more likely to be seen as mentally ill b ecause the defining of illness is mainly done by males. * Link and Phelan (1995) reviewed all the evidence and concluded that research pointed out a relationship between low levels of deprivation and mental illness.Social capital (Putnam 2000) argued that people who have extensive and strong levels of social networks are more likely to be ‘happier’ than those who don’t. Check Your Understanding: 1) The two sociological approaches to explaining mental illness are social realist and social constructionist. 2) Social realist bases itself on the idea that there are distinctive sets of abnormal behaviour that should be treated; they are very similar to the bio-medical approach.Social constructionist believes that definitions of mental illness can vary over time and in different cultures. 3) Labelling helps us understand that some people may live up to the label they have been given and can strip mental health patients of any self-dignity as they have no say in what ha ppens to them as they have been given the label of someone who is mad. 4) A structural explanation is closely tied to the social realist definition of mental illness; they accept the reality of mental illness and set out to discover what social factors help cause them. ) Busfield says that it is true that some groups are more likely to find their behaviour defined as mental illness, compared to the behaviour of other groups however al of those groups experience higher levels of stress so mental illness would be higher. 6) They are more likely to show cultural characterises that are not seen as normal in a wider society. 7) They use the idea that women have more responsibilities than men and that the defining of mental illness tends to be dominated by male health professionals.

All Things Being Equal Essay - 1062 Words

All Things Being Equal Most of us have heard Martin Luther Kings I Have a Dream speech, or part of it, at some point in our lives. In 1964, one year after the speech was given, Lyndon B. Johnson signed Executive Order 11246, which signaled the birth of affirmative action. Affirmative action refers to efforts to increase educational and employment opportunities for minorities and women. In November, 1997, California voters did away with affirmative action [at the state level] by passing proposition 209. They were convinced that it was no longer needed, that it gave minorities and women special rights. Governor Pete Wilson wrote in 1996 as part of his campaign against affirmative action: Let me be clear: we havent yet fully†¦show more content†¦We are struggling over crumbs because we know that the bigger part hs been received by the elites in corporate America. (Brunk 331) An example of this fighting over crumbs is the California proposition 209. White people felt they were not getting jobs, scholarships, into college, because the jobs, scholarships, etc., were being given to less qualified people of color. Some people of color were also against affirmative action because they didnt feel like they made it on their own accord but were hired or admitted to college, etc., because of their skin color and not because of their merits. This inferiority complex is shared by Richard Rodriguez, an American essayist and teacher. According to the biographical sketches in Literacies, Despite his academic success, Rodriguez left the university partly because he was uncomfortable with advantages he felt he received because of his minority status. (Brunk 697) To understand affirmative action we must look back in history. According to Cornel West,The fundamental aim of affirmative action was to put a significant dent in the tightly controlled networks of privileged white citizens who monopolized the good jobs and influential positions in American society. (Curry 32) Affirmative action is not aboutShow MoreRelatedAll Things Not Being Equal827 Words   |  4 PagesAll Things Not Being Equal An ongoing debate continues in the academic and scientific world of psychology in regards to the measurement of hypotheses, theories, and phenomenon. For the researcher, the argument is worrisome as well as tedious in nature. Most have the desire for the greatest statisticians to arrive at a consensus or standard, and allow the remainder to return to research business as usual. Very few if any researchers enjoy, comprehend, or desire to be knee deep in what a p value reallyRead MoreAnalysis Of Animal Farm, By George Orwell1235 Words   |  5 Pagesthe Soviet Union. Animal farm includes use of propaganda as well. 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